Separation Science Glycan Analysis offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for analytical scientists characterizing therapeutics based on glycan profiles and glycosylation.
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Biopharma Applications Compendium
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Fast profiling N-glycans in biotherapeutic antibodies by UHPLC-FLD with MS confirmation
Stefan Mittermayr (NIBRT, Ireland)
The development and optimization of a widely applicable UHPLC approach to fast, comprehensive profiling of 2-AA and 2-AB labeled glycans in IgG antibodies. The approach was validated with a human serum IgG and a commercial chimeric IgG1 mAb (infliximab). Glycan profiles were confirmed by exoglycosidase enzyme digestion and high-resolution, accurate-mass mass spectrometry, allowing increased sample throughput.
An ultrafast, batch-to-batch comparison of monoclonal antibody glycosylation
To develop a high-throughput screening method using HILIC UHPLC separation of 2AA-labelled glycans in a model mAb as a proof of concept for a more general approach to mAb glycoprofiling capable of identifying differences in high abundance gylcoforms.
Biopharmaceutical Application Notebook
A compilation of more than twenty application notes covering important aspects of biopharmaceutical analysis such as glycosylation and determination of drug-to-antibody ratio (DAR) of ADCs. The last section of the notebook provides tips and tricks to improve chromatographic separation of biomolecules.
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